Journal: Journal of Cell Communication and Signaling

Article Title: METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit

doi: 10.1007/s12079-022-00687-x

Figure Lengend Snippet: METTL13 modulated HN1L expression in GC. a Genes that showed positively association with METTL13 were surveyed by COEXPEDIA database ( http://www.coexpedia.org/ ). qRT-PCR was performed to test the expression of METTL13 and HN1L in METTL13 knockdown cells ( b ) and METTL13 overexpression cells ( c ). d HN1L or METTL13 mRNA expression was upregulated in GC tissues than that in the normal tissues based on GEPIA online analysis for TCGA database. e Positive correlation between METTL13 and HN1L expression at mRNA levels were analyzed using GEPIA online tools. f Western blotting was applied to test HN1L protein level upon METTL13 knockdown or overexpression in GC cells. g CCK-8 assays showed that cell viability caused by overexpression of METTL13 was partially attenuated by introducing siRNA targeting HN1L into SCG7901 cells. Data are given as mean ± SD. * P < 0.05; ** P < 0.01

Article Snippet: The primary antibodies used in this study are as follows: METTL13 (#ab186002, Abcam), HN1L (#ab200571, Abcam), eEF1A (#2551, Cell Signaling Technology), β-actin (#81178, Santa Cruz Biotechnology).

Techniques: Expressing, Quantitative RT-PCR, Knockdown, Over Expression, Western Blot, CCK-8 Assay

Journal: Journal of Cell Communication and Signaling

Article Title: METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit

doi: 10.1007/s12079-022-00687-x

Figure Lengend Snippet: eEF1A was involved in the regulation of HN1L by METTL13. a Knockdown efficacy for eEF1A1 or eEF1A2 was verified by qRT-PCR in AGS and MGC803 cells. b HN1L expression was analyzed by qRT-PCR in eEF1A1 or eEF1A2 knockdown cells. c Mutation site of eEF1A1/eEF1A2. d mRNA level of eEF1A1 or eEF1A2 was tested by qRT-PCR in SGC7901 cells transfected with pENTER-eEF1A1 or pENTER-eEF1A2 plasmid. And overexpression of eEF1A1 or eEF1A2 led to expression upregulation of HN1L by qRT-PCR analysis. No obvious change of HN1L expression was observed between wild type eEF1A and eEF1A K55A mutant cells. e Western blotting indicated that HN1L was decreased when eEF1A1 or eEF1A2 was silenced. f Western blot results were shown, indicating both wild-type and K55A mutant eEF1A upregulated HN1L expression. g Knockdown of eEF1A could partially offset the upregulation of HN1L in METTL13-overexpressed cells. Data are given as mean ± SD. * P < 0.05; ** P < 0.01

Article Snippet: The primary antibodies used in this study are as follows: METTL13 (#ab186002, Abcam), HN1L (#ab200571, Abcam), eEF1A (#2551, Cell Signaling Technology), β-actin (#81178, Santa Cruz Biotechnology).

Techniques: Knockdown, Quantitative RT-PCR, Expressing, Mutagenesis, Transfection, Plasmid Preparation, Over Expression, Western Blot

Journal: Cell Reports Medicine

Article Title: The Fc-effector function of COVID-19 convalescent plasma contributes to SARS-CoV-2 treatment efficacy in mice

doi: 10.1016/j.xcrm.2022.100893

Figure Lengend Snippet:

Article Snippet: Peroxidase AffiniPure Goat Anti-Human IgA + IgG + IgM (H + L) , Jackson ImmunoResearch , 109-035-064.

Techniques: Blocking Assay, Control, Virus, Variant Assay, Recombinant, Modification, Saline, Red Blood Cell Lysis, Lysis, Electron Microscopy, Staining, Luciferase, Multiplex Assay, cDNA Synthesis, Oligo Synthesis, Plasmid Preparation, Expressing, Software, Flow Cytometry, Real-time Polymerase Chain Reaction, Spectrophotometry, Membrane, Stripping Membranes, Isolation, In Vivo Imaging